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Amylase
Investigation Result Units Biological Reference Interval

Amylase Enzymatic 193 U/L 28 - 100

• Sample type : Serum. • Method : Enzymatic Colorimetric assay. • Note : Amylase is produced in the Pancreas and most of the elevation in serum is due to increased rate of Amylase entry into the blood stream / decreased rate of clearance or both. Serum Amylase rises within 6 to 48 hours of onset of Acute pancreatitis in 80% of patients, but is not proportional to the severity of the disease. Activity usually returns to normal in 3-5 days in patients with milder edematous form of the disease. Values persisting longer than this period suggest continuing necrosis of pancreas or Pseudocyst formation. Approximately 20% of patients with Pancreatitis have normal or near normal activity. Hyperlipemic patients with Pancreatitis also show spuriously normal Amylase levels due to suppression of Amylase activity by triglyceride. Low Amylase levels are seen in Chronic Pancreatitis, Congestive Heart failure, 2nd & 3rd trimesters of pregnancy, Gastrointestinal cancer & bone fractures.

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Lipase
Investigation Result Units Biological Reference Interval

Lipase 20.6 U/L 13 - 60

• Sample Type : Serum. • Method: Quinone Dye. • Comments : Pancreas is the major and primary source of serum lipase though lipases are also present in liver, stomach, intestine, WBC, fat cells and milk. In acute pancreatitis, serum lipase becomes elevated at the same time as amylase and remains high for 7-10 days. Increased lipase activity rarely lasts longer than 14 days. Prolonged increase suggests poor prognosis or presence of a cyst. The combined use of serum lipase and serum amylase is effective in ruling out acute pancreatitis. Increased levels are seen in Acute & Chronic pancreatitis, Obstruction of pancreatic duct, Non pancreatic conditions like renal diseases, acute cholecystitis, intestinal obstruction, duodenal ulcer, alcoholism, diabetic ketoacidosis and following endoscopic retrograde cholangiopancreatography.

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